33g steel needle  (World Precision Instruments)

Journal: bioRxiv

Article Title: Medial septum neurokinin- and somatostatin-sensitive mechanisms mediate sensorimotor and nociceptive behaviours

doi: 10.1101/2020.10.27.358267

Figure Lengend Snippet: (A) Representative microinjection site in the MS. The drug was microinjected into the MS via a single 33G microinjection stainless steel needle coupled to a microsyringe. (B) Composite representation of microinjection sites in MS built as explained in . Formalin (5%, 0.05ml) was injected 15 min after microinjection of NK1R antagonist, L-733,060 (1x, 0.0176 μg/μl, n = 8; 10x, 0.176 μg/μl, n = 7), or vehicle during period of large irregular hippocampal field activity (LIA). (C) Microinjection of L-733,060 into MS attenuated formalin-induced suppression of CA1 PS. The PS traces on the left and histograms are built as explained in . (D) Histograms illustrating lack of effect of L-733,060 pre-treatment on fast Fourier transform (FFT; resolution of 0.5 Hz) parameters of hippocampal theta wave activity (FFT theta peak power, left and FFT theta peak frequency, right). FFT analysis was performed on theta wave activity recorded in the first 5 min after formalin injection. The FFT theta peak power was normalised to spontaneous theta power recorded prior to the experiment. Data presented are mean ± S.E.M. Significant difference (p < 0.05): * vs. Vehicle MS; two-tailed unpaired t-test.

Article Snippet: The drugs were administered into the MS either via a 33G stainless steel microinjection needle attached to an Exmire microsyringe (Ito Corporation, Fuji, Japan) or via a double-barrelled cannula system.

Techniques: Injection, Activity Assay, Two Tailed Test